AtNaR Nitrate Reductase Reagents Kit for High Capacity Discrete Analyzers - Two tubes with yellow and red caps labeled 'NADH' and 'AtNaR', and a vial of enzyme diluent
AtNaR Nitrate Reductase Reagents Kit for Standard Discrete Analyzers - Two tubes with yellow and green caps labeled 'NADH' and 'AtNaR', and a vial of enzyme diluent

NECi

AtNaR Nitrate Reductase Reagents Kit for Discrete Analyzers

$90.00
SKU: NRPk-HDA-3At
Quantity:High Capacity Kit for 300+ Assays
Quantity:

EPA-Compliant, Cadmium-Free Nitrate Reagent Kit for Discrete Analyzers

AtNaR Nitrate Reductase Reagent Packs were developed by NECi in conjunction with the United States Geological Survey (USGS) to replace toxic cadmium used in automated colorimetric nitrate determination methods. The enzymatic nitrate reduction approach aligns with procedures referenced by the U.S. Environmental Protection Agency (EPA) under 40 CFR Part 136 for nitrate determination in wastewater and environmental water samples. This method is also included in the California Environmental Laboratory Accreditation Program (ELAP) Field of Accreditation (FOA) tables, enabling laboratories to pursue accreditation using this cadmium-free method.

This non-toxic, environmentally benign alternative provides reliable nitrate reduction using water-soluble reagents optimized for discrete analyzers.

Product Format

AtNaR Nitrate Reductase Reagent Pack for Discrete Analyzers

  • Animal origin free
  • Designed for ThermoFisher Gallery discrete analyzers
  • May be compatible with other discrete analyzers with similar specifications
  • This kit contains only enzyme and co-factor. You will also need assay buffer and Griess reagents to run the assay. 

Reaction Principle

AtNaR catalyzes the reduction of nitrate (NO₃⁻) to nitrite (NO₂⁻) using NADH as the electron donor.
The generated nitrite can then be reacted with Griess reagents and quantified using standard colorimetric detection methods.

Key Features

  • Safe & Non-Toxic: Eliminates hazardous cadmium from your laboratory workflow
  • Environmentally Friendly: All reagents are water-soluble and environmentally benign
  • Exceptional Stability: Up to 6 months shelf life at room temperature, highly stable in working solution
  • Superior Performance: Highest specific activity AtNaR on the market with high specificity to reduce false positives
  • Wide Operating Range: Effective for analysis run between 20 – 45°C
  • Salt Tolerant: Suited for 3%+ NaCl environments
  • Cost-Effective: Stable working solutions minimize waste and reagent consumption
  • Quality Assured: Recombinant enzyme manufactured in Pichia pastoris under strict QA/QC procedures for lot-to-lot consistency and reliability

Product Specifications

  • CAS Number: 9013-03-0
  • Physical Form: Freeze-dried protein glass
  • Specific Activity: 5-15 units/mg enzyme protein
  • Expected Purity Range: Greater than 95%
  • Temperature Range in Assay: 15-45°C
  • Manufacturing: Recombinantly manufactured in Pichia pastoris

Applications

Ideal for laboratories performing nitrate analysis in:

  • Drinking water quality testing and regulatory compliance
  • Environmental monitoring and surface water analysis
  • Wastewater treatment facility testing
  • Groundwater contamination assessment
  • Agricultural runoff monitoring
  • Research and development laboratories
  • Government and regulatory testing facilities

Analyzer Compatibility

These kits are designed for ThermoFisher Gallery discrete analyzers and may be compatible with other discrete analyzers with similar specifications. Please inquire about reagent packs with detailed instructions for other discrete analyzers, continuous and segmented flow analyzers, and other automated laboratory instruments.

Kit Contents

Standard DA Kit for 75 assays:

  • 1.0 unit AtNaR (freeze-dried)
  • 2.0 mg NADH (solid form)
  • 1 vial enzyme diluent

High Capacity DA Kit for 300+ assays:

  • 3.0 units AtNaR (freeze-dried)
  • 2.0 mg NADH (solid form)
  • 1 vial enzyme diluent

Storage Conditions & Shelf Life Information*

*Shelf life guaranteed if stored as directed below


 -80°C -20°C 4°C RT: 20°C – 25°C Above 40°C
Freeze-Dried 3+ Years 2+ Years 1+ Year 6 months Up to 1 week
In Solution* 1+ Year 6+ months Up to 1 week Not recommended Not recommended

* Enzyme provided with enzyme diluent working solution, contains 25% glycerol

Compatible Products

Ordering Information

Contact us to inquire about bulk quantity discounts or wholesale orders, or to request a quote for international orders.

Please inquire about reagent packs with detailed instructions for other discrete analyzers, continuous and segmented flow analyzers, and other automated laboratory instruments.

Resources & Downloads

Nitrate Reductase Reagent Pack for Tabletop Discrete Analyzers

Nitrate Reductase Reagent Pack for High Capacity Discrete Analyzers

SDS for Reagent Packs

Nitrate Reductase Storage and Reconstitution