The enzymatic methods behind our products are grounded in decades of scientific research. Wilbur H. Campbell's academic career spanned from the late 70's to 2003 and he published over 125 peer-reviewed articles over that span. NECi was founded in 2003 and there's a reduced publication stream from then on. The selection of publications below represent work on recombinant protein expression and analytical applications.
Peer-Reviewed Journal Articles
"Enzyme-Catalyzed O2 Removal System for Electrochemical Analysis under Ambient Air: Application in an Amperometric Nitrate Biosensor." Analytical Chemistry, 2012.
"Construction and Characterization of Nitrate Reductase-Based Amperometric Electrode and Nitrate Assay of Fertilizers and Drinking Water." Analytical Chemistry, 1998.
"Nitrate Reductase Structure, Function and Regulation: Bridging the Gap between Biochemistry and Physiology." Annual Review of Plant Physiology and Plant Molecular Biology, 1999.
"Reversible Light/Dark Modulation of Spinach Leaf Nitrate Reductase Activity Involves Protein Phosphorylation." Archives of Biochemistry and Biophysics, 1992.
"Affinity Binding via Zinc(II) for Controlled Orientation and Electrochemistry of Histidine-Tagged Nitrate Reductase in Self-Assembled Monolayers." Bioelectrochemistry, 2013.
"Nitrate Reductase for Nitrate Analysis in Water." Environmental Chemistry Letters, 2006.
"Corn Leaf Nitrate Reductase: A Nontoxic Alternative to Cadmium for Photometric Nitrate Determinations in Water Samples by Air-Segmented Continuous-Flow Analysis." Environmental Science and Technology, 2002.
"Genetic Modification of Pichia pastoris for Production of Propylene Glycol from Glycerol." International Journal of Genetic Engineering, 2012.
"Viscosity Effects on Eukaryotic Nitrate Reductase Activity." Journal of Biological Chemistry, 2005.
"Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method." MethodsX, 2015.
"Non-Toxic Total Nitrogen Determination Using a Low Alkaline Persulfate Digestion." MethodsX, 2020.
"Reduction of Nitrate and Nitrite in Water by Immobilized Enzymes." Nature, 1992.
"Higher Plant Responses to Environmental Nitrate." Physiologia Plantarum, 1991.
"Nitrate Reductase and Its Role in Nitrate Assimilation in Plants." Physiologia Plantarum, 1988.
"Structural Basis of Eukaryotic Nitrate Reduction: Crystal Structures of the Nitrate Reductase Active Site." The Plant Cell, 2005.
"Recombinant Expression of Molybdenum Reductase Fragments of Plant Nitrate Reductase at High Levels in Pichia pastoris." Plant Physiology, 2000.
"Analysis of Wild-Type and Mutant Plant Nitrate Reductase Expressed in the Methylotrophic Yeast Pichia pastoris." Plant Physiology, 1997.
"Nitrate Reductase Biochemistry Comes of Age." Plant Physiology, 1996.
"Codon Usage in Higher Plants, Green Algae, and Cyanobacteria." Plant Physiology, 1990.
"Regulation of Corn Leaf Nitrate Reductase." Plant Physiology, 1986.
"Open-Source Photometric System for Enzymatic Nitrate Quantification." PLOS ONE, 2015.
"Purification and Biochemical Characterization of Simplified Eukaryotic Nitrate Reductase Expressed in Pichia pastoris." Protein Expression and Purification, 2004.
"Functional Domains of Assimilatory Nitrate Reductases and Nitrite Reductases." Trends in Biochemical Sciences, 1990.
Government Reports
"Analytical Properties of Some Commercially Available Nitrate Reductase Enzymes Evaluated as Replacements for Cadmium in Automated, Semiautomated, and Manual Colorimetric Methods for Determination of Nitrate Plus Nitrite in Water." U.S. Geological Survey Scientific Investigations Report, 2013.
Conference Proceedings
"Field Determination of Nitrate Using Nitrate Reductase." Air & Waste Management Association Symposium, 1997. (PDF)
Technical Reports
"TNT and RDX Degradation by Cell-Free Extracts of Clostridium acetobutylicum." U.S. Army Corps of Engineers, 1999. (PDF)