U.S. EPA Clean Water Act Methods Update Rule for the Analysis of Effluent |
ASTM International Standard Test Method for Nitrite-Nitrate in Water by Nitrate Reductase |
USGS Colorimetric Determination of Nitrate Plus Nitrite in Water by Enzymatic Reduction, Automated Discrete Analyzer Methods |
Standard Methods Inorganic Nonmetallic Constituents in Water and Wastewater |
The NELAC Institute Method Codes
These method codes are recognized by The NELAC Institute for certified nitrate testing under EPA and USGS standards.
| Method Code | Method Reference | Revision Date | Method Title |
|---|---|---|---|
| 40005285 | USGS I-2547-11 | 2011 | Standard Level Colorimetric Determination of Nitrate plus Nitrite by Enzymatic Reduction, Automated Discrete Analyzer Method |
| 40005296 | USGS I-2548-11 | 2011 | Low-Level Colorimetric Determination of Nitrate plus Nitrite by Enzymatic Reduction, Automated Discrete Analyzer Method |
| 30034107 | ASTM D7781-14 | 2014 | Standard Test Method for Nitrate-Nitrite in Water by Nitrate Reductase |
| 40 CFR Part 136 | EPA N07-0003 (Alternate to EPA Method 353.2) | 2017 | Determination of Nitrate plus Nitrite by Enzymatic Reduction, Automated Discrete Analyzer Method |
U.S. EPA Safe Drinking Water Act Reporting
- EPA 40 CFR Part 141 | Vol. 81, No. 138 | Expedited Approval of Alternative Test Procedures for the Analysis of Contaminants Under the Safe Drinking Water Act; Analysis and Sampling Procedures | Published July 19, 2016
- EPA SDWA Expedited Approval of Alternative Test Procedures Fact Sheet 2016
- Alternate Test Procedure Validation Report: Enzymatic Reduction Method of Nitrate-N Analysis in Drinking Water
- EPA SDWA Method for Nitrate Reductase Nitrate-Nitrogen Analysis of Drinking Water
U.S. EPA Clean Water Act Reporting
- Whitepaper: Enzymatic Nitrate Analysis for EPA Regulated Laboratories Using Nitrate Reductase
- Review of NECi Method N07-0003, Method for Nitrate Reductase Nitrate-Nitrogen Analysis | ATP Review Letter | April 4th, 2014 | U.S. EPA Office of Science and Technology
- EPA Alternate Test Procedure Validation Report for Enzymatic Reduction Method of Nitrate-N Analysis | CWA Reporting | Published February 20th, 2014
- U.S. EPA Federal Register | Clean Water Act Methods Update Rule for the Analysis of Effluent; Proposed Rule | Vol. 80, No. 33 | Published February 19, 2015
- U.S. EPA Federal Register | Clean Water Act Methods Update Rule for the Analysis of Effluent; Final Rule | Vol. 82, No. 165 | Published August 28, 2017
- U.S. EPA Clean Water Act Analytical Methods | Methods Update Rule 2017
- U.S. EPA Clean Water Act Analytical Methods | Alternate Test Procedures
ASTM International
- Nitrate Reductase Research Report | Committee D19 on Water, Subcommittee D19.05 on Methods for Analysis of Inorganic Substances in Water
- ASTM Method D7781-23 | Standard Test Method for Nitrite-Nitrate in Water by Nitrate Reductase
- ASTM Standardization News | New Proposed Nitrate Reductase Method
- California ELAP Field of Accreditation Table 108: Inorganic Constituents (Non-potable Water) — ASTM D7781-14 approved for wastewater and ambient water laboratories in California
U.S. Geological Survey
- Colorimetric Determination of Nitrate plus Nitrite in Water by Enzymatic Reduction, Automated Discrete Analyzer Methods
- Analytical Properties of Some Commercially Available Nitrate Reductase Enzymes Evaluated as Replacements for Cadmium in Automated, Semiautomated, and Manual Colorimetric Methods for Determination of Nitrate plus Nitrite in Water
- National Environmental Methods Index (NEMI) USGS-NWQL: I-2547-11: Standard Level Colorimetric Determination of Nitrate plus Nitrite in Water by Enzymatic Reduction, Automated Discrete Analyzer Method
- National Field Manual for the Collection of Water-Quality Data Chapter A4: Collection of Water Samples