Purine Nucleoside Phosphorylase (PNP)

The PNP Phosphate Assay is a drop-in replacement for EPA Method 365.3 — delivering accurate orthophosphate results without molybdate, antimony, acids, or multi-step reagent handling.

Using purified PNP enzyme + MESG substrate, the assay converts inorganic phosphate into a measurable absorbance shift in a single, clean endpoint reaction — no hazardous waste, no complex timing.

NECi's PNP Phosphate Assay delivers a modern, enzyme-driven approach to orthophosphate analysis. Using purified PNP enzyme and the MESG substrate, this method provides a simple, reliable endpoint reaction without the hazardous reagents, complex timing, or multi-step workflows required by legacy colorimetric chemistry.

• Cleaner and Simpler Than EPA Method 365.3
• More efficient — single-step endpoint reaction, no kinetic timing or multiple reagents
• No Hazardous Waste — eliminates molybdate, antimony and acids
• Safer for technicians — reduced chemical exposure and simpler handling
• Compatible with non-sulfate digestion methods

NECi's recombinant Purine Nucleoside Phosphorylase (PNP) replaces molybdenum/antimony testing with a safe, biodegradable, and non-toxic enzymatic method. Traditional phosphate measurement depends on toxic metals such as molybdenum and antimony. The PNP method is a drop-in replacement that simply replaces toxic heavy metal reagents with non-toxic biodegradable enzymes.

• No Molybdate — No Antimony — No Acids/Oxidizers
• No Hazardous Waste Disposal
• Lower Total Cost of Testing
• Supports Green Chemistry and Sustainable Water Testing

NECi's recombinant enzyme manufacturing process delivers exceptional purity, performance, and consistency — all critical qualities for certified laboratories, instrument manufacturers, researchers, and regulatory testing environments. PNP reduces analysis complexity and the need for multiple steps and reagents in a limited timeframe.

• End Point Reaction
• Single Catalyst
• Recombinant Enzymes for Reliable Performance
• Rigorous QA/QC From Start to Finish
• Guaranteed Stability and Performance

The PNP/MESG endpoint produces a clear absorbance shift measurable on any standard UV-Vis photometer (bench-top or portable), making it easy to integrate into existing laboratory workflows and instruments. It is easily compatible with any autoanalyzer making it ideal for high-throughput and automated platforms.

• Designed for Reliability in OEM Applications
• Compatible with UV-Vis and Portable Photometer Platforms
• Simpler Integration Than Molybdate-Based Methods

U.S.-manufactured with strict quality control — guaranteed activity, stability, and reproducibility, backed by decades of enzymology expertise. NECi backs all bulk shipments with robust QA documentation and full traceability.

• U.S.-Manufactured with Strict QA/QC
• Guaranteed Activity, Stability, and Reproducibility
• Full Traceability and QA Documentation for Bulk Orders
• Backed by Decades of Enzymology Expertise

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