Enzymatic Sugar Oxidation for Analytical Applications
Pyranose Oxidase (PyrOx) is an enzyme that oxidizes specific sugars, allowing them to be quantified colorimetrically. This controlled enzymatic conversion enables sensitive optical detection in coupled oxidase–peroxidase assay systems. By leveraging molecular specificity under mild aqueous conditions, PyrOx supports accurate, reproducible signal generation across research, biosensor, and automated analytical platforms.
Substrate Specificity
PyrOx acts on a defined set of pyranose sugars, making it well-suited for selective detection in complex sample matrices. The table below shows kinetic parameters for NECi's recombinant PyrOx, as reported in the article linked below:
| Substrate | vmax (μmol min⁻¹ mg⁻¹) | Km (mM) | kcat (s⁻¹) | kcat/Km (mM⁻¹ s⁻¹) |
|---|---|---|---|---|
| d-Glucose | 19.9 ± 0.74 | 0.84 ± 0.15 | 83.1 ± 3.1 | 98.9 |
| l-Sorbose | 14.1 ± 0.81 | 23.5 ± 3.4 | 58.8 ± 3.4 | 2.50 |
| d-Xylose | 10.8 ± 0.48 | 20.9 ± 2.7 | 44.9 ± 2.0 | 2.15 |
| d-Galactose | 1.17 ± 0.03 | 2.94 ± 0.35 | 4.87 ± 0.13 | 1.66 |
| d-Fructose | 0.45 ± 0.068 | 103 ± 46 | 1.88 ± 0.28 | 0.0183 |
This substrate range supports multi-analyte flexibility within a single enzyme platform, enabling developers to target specific sugars depending on assay design and sample type.
Recombinant Production
NECi utilizes recombinant enzyme technology to produce Pyranose Oxidase with defined sequence identity and consistent performance. Recombinant production offers several important advantages:
- Defined enzyme identity and sequence
- High purity and reduced background proteins
- Reliable batch-to-batch consistency
- Scalable production to support analytical demand
Interested in Pyranose Oxidase?
NECi offers recombinant Pyranose Oxidase for research and analytical development. This enzyme is supplied as a standalone component rather than a packaged kit, and is available in liquid or lyophilized formats. Custom bulk quantities are available for OEM and commercial applications.
For pricing, specifications, or technical discussion:
Scientific Literature
Ines Pisanelli, Magdalena Kujawa, Oliver Spadiut, Roman Kittl, Petr Halada, Jindrich Volc, Michael D. Mozuch, Philip Kersten, Dietmar Haltrich, Clemens Peterbauer,
Pyranose 2-oxidase from Phanerochaete chrysosporium—Expression in E. coli and biochemical characterization,
Journal of Biotechnology,
Volume 142, Issue 2,
2009,
Pages 97-106,
ISSN 0168-1656,
https://doi.org/10.1016/j.jbiotec.2009.03.019