The US EPA has included NECi's enzyme-based nitrate method in the 2015 Methods Update Rule. This new method is for Clean Water Act reporting (40 CFR Part 136) of nitrate/nitrite in ground and waste water samples. In the new method, recombinant Nitrate Reductase enzyme replaces Cadmium for the nitrate reduction step. Enzyme-based nitrate determination can be used in any system based on Griess reagent chemistry and spectrophotometric detection. 

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