NECi
PNP - Purine Nucleoside Phosphorylase (Enzyme Only)
Choose options
Recombinant PNP for Precise Phosphate Quantification
NECi's recombinant Purine Nucleoside Phosphorylase (PNP) is a highly purified enzyme used for accurate phosphate quantification in laboratory applications. It catalyzes the orthophosphate-dependent transfer to ribose with release of free purine base, providing a reliable and specific method for phosphate detection.
This enzyme is ideal for researchers and laboratories requiring precise phosphate measurements with minimal sample preparation and high specificity to reduce false positives.
Product Format
Purine Nucleoside Phosphorylase (Enzyme Only)
- Affinity purified recombinant enzyme
- Animal origin free
- Supplied as freeze-dried protein glass with enzyme diluent bulb
- Available in 1, 2, 3, and 5 unit quantities
Enzyme Activity
One unit produces 1 µmole of hypoxanthine from inosine per minute at pH 8.0 and 22°C.
Key Features
- High Specificity: Reduces false positives in phosphate detection
- MESG Co-factor: Utilizes MESG (2-amino-6-mercapto-7-methylpurine riboside) as the co-factor to accurately quantify phosphate in samples
- Minimal Sample Preparation: Little sample preparation required for analysis
- Small Sample Size: Requires small sample size in relation to total assay volume
- Consistent & Reliable: Recombinant production ensures lot-to-lot consistency
- Quality Assured: Affinity purified for maximum enzyme activity and purity
Product Specifications
- CAS Number: 9030-21-1
- Physical Form: Freeze-dried protein glass
- Specific Activity: 5-15 units/mg protein
- Expected Purity Range: Greater than 95%
- Temperature Range in Assay: 15-45°C
- Manufacturing: Recombinantly manufactured in Escherichia coli
Applications
Ideal for laboratories performing phosphate analysis in:
- Biochemical research and enzyme kinetics studies
- Phosphate quantification assays
- Metabolic pathway analysis
- Drug discovery and development
- Quality control testing
- Academic and industrial research laboratories
Storage Conditions & Shelf Life Information*
*Shelf life guaranteed if stored as directed below
| -20°C or lower | 4°C | RT: 20°C – 25°C | 30-40°C | |
|---|---|---|---|---|
| Freeze-Dried (vacuum-sealed packets) | Stable indefinitely | 24+ months | 12+ months | 1 week |
| Freeze-Dried (in vials, desiccated) | 12+ months | 12+ months | - | - |
| Working Solution (1 U/ml in buffer) | 6 - 12 months | 1 week | >12 hours | - |
Ordering Information
Contact us to inquire about bulk quantity discounts or wholesale orders, or to request a quote for international orders.
Resources & Downloads
PNP Storage and Reconstitution
PNP Specification Sheet *Coming Soon*