Microplate Format Nitrate Test Kit - Low Range saltwater kit with 96-well plate, nitrate reductase, NADH, assay buffer, NaCl water, and color reagents
Microplate Format Nitrate Test Kit - Low Range freshwater kit with 96-well plate, nitrate reductase, NADH, assay buffer, and color reagents
Microplate Format Nitrate Test Kit - Standard Range saltwater kit with 96-well plate, nitrate reductase, NADH, assay buffer, NaCl water, and color reagents
Microplate Format Nitrate Test Kit - Standard Range freshwater kit with 96-well plate, nitrate reductase, NADH, assay buffer, and color reagents

NECi

Microplate Format Nitrate Test Kits

$130.00
SKU: NTK-MPLR
Range:Low Range: 0.05 - 1.0 ppm Nitrate-N
Freshwater or Saltwater:Freshwater
Quantity:

Quantitative Nitrate Analysis for High-Throughput Testing

NECi's Microplate Format Nitrate Test Kit delivers high-throughput nitrate quantification in any aqueous sample using a 96-well microplate. The enzyme-based assay uses recombinant Nitrate Reductase (NaR) to reduce nitrate to nitrite via NADH, which then reacts with color reagents to produce a measurable signal — read at 540 nm against nitrate standards. No cadmium reduction required.

Available Ranges

*Nitrate is reported in different units depending on your field of use and where you live.

NTK Microplate Kits ppm Nitrate-N ppm Nitrate µM Nitrate
Low Range Kit 0.05 - 1.0 0.2 - 4.4 3.6 - 71.4
Standard Range Kit 0.5 - 10.0 2.0 - 44 36 - 714

Kit Options

  • Choose either Low Range or Standard Range
  • Choose either Freshwater or Saltwater Analysis

Materials You Will Need

You will need a microplate reader capable of reading at 540nm ± 20nm, variable pipetters (multichannel recommended), nitrate-free deionized water, concentrated HCl, and ice.

Kit Contents

  • 96-Well Microplate
  • Assay Buffer
  • Color Reagent No. 1 Powder (Sulfanilamide)
  • Color Reagent No. 2 Powder (N-(1-Naphthyl)ethylenediamine)
  • Freeze Dried NADH
  • Freeze Dried Nitrate Reductase
  • Enzyme Diluent
  • 100 ppm Nitrate-N Standard
  • Microcentrifuge Tubes
  • Multi-channel Pipette Reagent Reservoir
  • Set of Instructions
  • Nitrate-free Salt Water (only included in saltwater kits)

Key Features

  • Animal origin free
  • Recombinant enzyme produced according to strict QA/QC procedures, ensuring lot-to-lot consistency, reliability, and stability
  • Purified by Immobilized Metal Affinity Chromatography (IMAC) guaranteeing greater than 95% purity
  • Small sample volume and enzyme reagents offer sustainability and no-hassle shipping, storage, and disposal for your laboratory

Applications

  • Wastewater and environmental water quality monitoring
  • Soil and plant extract nitrate analysis
  • Agricultural and food testing
  • Research and quality control

Storage Conditions & Shelf Life Information*

*Shelf life guaranteed if stored as directed below


 -80°C -20°C 4°C RT: 20°C – 25°C Above 40°C
Freeze-Dried 3+ Years 2+ Years 1+ Year 6 months Up to 1 week
In Solution* 1+ Year 6+ months Up to 1 week Not recommended Not recommended

* Enzyme provided with enzyme diluent working solution, contains 25% glycerol

Ordering Information

Customers in the United States and Canada can order directly through our website. Contact us for bulk quantity discounts, wholesale orders, or shipping quotes to other countries.

Resources & Downloads

Low Range Microplate Format Nitrate Test Kit Instructions

Standard Range Microplate Format Nitrate Test Kit Instructions

SDS for Test Tube Format and Microplate Format Nitrate Test Kits