Benchtop Nitrate Test Kit - Low Range freshwater kit with nitrate reductase, NADH, assay buffer, and color reagents
Benchtop Nitrate Test Kit - Standard Range freshwater kit with nitrate reductase, NADH, assay buffer, and color reagents
Benchtop Nitrate Test Kit - Saltwater kit with nitrate reductase, NADH, assay buffer, NaCl water, and color reagents
Benchtop Nitrate Test Kit - Standard Range freshwater kit with nitrate reductase, NADH, assay buffer, and color reagents
Benchtop Nitrate Test Kit - Standard Range freshwater kit with nitrate reductase, NADH, assay buffer, and color reagents
Benchtop Nitrate Test Kit - Saltwater kit with nitrate reductase, NADH, assay buffer, NaCl water, and color reagents
Benchtop Nitrate Test Kit - Saltwater kit with nitrate reductase, NADH, assay buffer, NaCl water, and color reagents
Benchtop Nitrate Test Kit - Saltwater kit with nitrate reductase, NADH, assay buffer, NaCl water, and color reagents
Benchtop Nitrate Test Kit - Low Range freshwater kit with nitrate reductase, NADH, assay buffer, and color reagents
Benchtop Nitrate Test Kit - Low Range freshwater kit with nitrate reductase, NADH, assay buffer, and color reagents
Benchtop Nitrate Test Kit - Saltwater kit with nitrate reductase, NADH, assay buffer, NaCl water, and color reagents

NECi

Benchtop Nitrate Test Kit

$50.00
SKU: NTK-TTSR-25
Quantity:25 Sample Analysis
Range:Standard Range: 0.5 ppm - 10.0 ppm Nitrate-N
Fresh or Saltwater:Freshwater
Quantity:

Quantitative Nitrate Analysis for Laboratory Use

NECi's Benchtop Nitrate Test Kit delivers precise, enzyme-based nitrate quantification for any aqueous sample — from wastewater to soil and plant extracts. The test tube format uses recombinant Nitrate Reductase (NaR) to reduce nitrate (NO₃⁻) to nitrite (NO₂⁻) via NADH, which is then quantified colorimetrically at 540 nm ± 20 nm using your colorimeter or spectrophotometer. No toxic cadmium reduction required.

Available Ranges

Nitrate is reported in different units depending on your field of use and where you live.

NTK Test Tube Kits ppm Nitrate-N ppm Nitrate µM Nitrate
Standard Range Kit 0.5 - 10.0 2.0 - 44 36 - 714
Low Range Kit 0.05 - 1.0 0.2 - 4.4 3.6 - 71.4

Kit Options

  • 25 Sample or 100 Sample Analysis
  • Choose either Freshwater or Saltwater Analysis
  • Choose either Standard Range or Low Range

Enzyme Variants

  • Standard Range Freshwater Kit: Contains YNaR (Yeast Nitrate Reductase)
  • All other variations (Standard Range Saltwater, Low Range Freshwater, Low Range Saltwater): Contain AtNaR (Arabidopsis thaliana Nitrate Reductase)

Both enzymes provide the same high-quality, reliable results with the key features listed below.

Materials You Will Need

You will need a graduated cylinder, variable pipetters, a test tube vortex-type mixer, colorimeter or spectrophotometer with glass or plastic cuvettes, clean and nitrate-free 13 x 100mm test tubes, a timer, nitrate-free deionized water, concentrated HCl, and ice.

Kit Contents

  • Assay Buffer
  • Color Reagent No. 1 Powder (Sulfanilamide)
  • Color Reagent No. 2 Powder (N-(1-Naphthyl)ethylenediamine)
  • Freeze Dried NADH
  • Freeze Dried Nitrate Reductase
  • Enzyme Diluent
  • 100 ppm Nitrate-N Standard
  • Microcentrifuge Tubes
  • Set of Instructions
  • Nitrate-free Salt Water (only included in saltwater kits)

Key Features

  • Animal origin free
  • Recombinant enzyme produced according to strict QA/QC procedures, ensuring lot-to-lot consistency, reliability, and stability
  • Purified by Immobilized Metal Affinity Chromatography (IMAC) guaranteeing greater than 95% purity
  • Small sample volume and enzyme reagents offer sustainability and no-hassle shipping, storage, and disposal for your laboratory

Applications

  • Wastewater and environmental water quality monitoring
  • Soil and plant extract nitrate analysis
  • Agricultural and food testing
  • Research and quality control

Storage Conditions & Shelf Life Information*

*Shelf life guaranteed if stored as directed below


 -80°C -20°C 4°C RT: 20°C – 25°C Above 40°C
Freeze-Dried 3+ Years 2+ Years 1+ Year 6 months Up to 1 week
In Solution* 1+ Year 6+ months Up to 1 week Not recommended Not recommended

* Enzyme provided with enzyme diluent working solution, contains 25% glycerol

Ordering Information

Contact us to inquire about bulk quantity discounts or wholesale orders, or to request a quote for international orders.

Resources & Downloads

Standard Range Test Tube Format Nitrate Test Kit Instructions: 25 Samples

Low Range Test Tube Format Nitrate Test Kit Instructions: 25 Samples

Standard Range Test Tube Format Nitrate Test Kit Instructions: 100 Samples

Low Range Test Tube Format Nitrate Test Kit Instructions: 100 Samples

SDS for Test Tube Format and Microplate Format Nitrate Test Kits