AtNaR Nitrate Reductase (Enzyme Only)

EPA-Compliant, Cadmium-Free Nitrate Reductase Enzyme for Laboratory Analysis

AtNaR Nitrate Reductase (Arabidopsis thaliana) was developed by NECi in conjunction with the United States Geological Survey (USGS) to replace toxic cadmium used in automated colorimetric nitrate determination methods. The enzymatic nitrate reduction approach aligns with procedures referenced by the U.S. Environmental Protection Agency (EPA) under 40 CFR Part 136 for nitrate determination in wastewater and environmental water samples.

This non-toxic, environmentally benign alternative provides reliable nitrate reduction using water-soluble reagents optimized for laboratory analysis.

Product Format

AtNaR Nitrate Reductase (Enzyme Only)

Supplied as a freeze-dried protein glass with enzyme diluent bulb
Animal origin free
Custom unit sizes available upon request

Reaction Principle

AtNaR catalyzes the reduction of nitrate (NO₃⁻) to nitrite (NO₂⁻) using NADH as the electron donor.
The generated nitrite can then be quantified using standard colorimetric detection methods.

One unit will reduce 1.0 μmole of nitrate to nitrite per minute in an NADH system at pH 7 ± 0.5 at 30°C.

Key Features

Safe & Non-Toxic: Eliminates hazardous cadmium from your laboratory workflow
Environmentally Friendly: All reagents are water-soluble and environmentally benign
Exceptional Stability: Up to 6 months shelf life at room temperature, highly stable in working solution
Superior Performance: High specific activity AtNaR with high substrate specificity to reduce false positives
Wide Operating Range: Effective for analysis run between 20 – 45°C
Salt Tolerant: Suited for 3%+ NaCl environments
Quality Assured: Recombinant enzyme manufactured in Pichia pastoris under strict QA/QC procedures for lot-to-lot consistency and reliability
Small sample size in relation to total assay volume
Little sample preparation required
No inhibition by organic or humic acids