Product ID: PTK-TTLR or PTK-TTSR

This kit is supplied complete with all necessary reagents except for HPLC water.  Use this kit for quantitative phosphate analysis of essentially any aqueous sample, from wastewater to soil and plant extracts.  Simply extract solid samples using HPLC water before analysis.  This test tube format kit is to be used with your UV capable spectrophotometer capable of reading absorbance at 360nm.    

Quantitates Phosphate in Complex Mixtures

Choose kit type using choices below, or contact us for a custom kit fit for your application:

• Standard Range (5 ppm - 20 ppm Phosphate) or Low Range (0.5 - 5 ppm Phosphate)

• Reagents for 25 Samples or 100 Samples

When reaction is complete, the purine product yields an increase in absorbance at 360 nm in equal molar ratio to the inorganic phosphate in the reaction mixture.  Using the equation of the linear regression line fitted to the set of phosphate standards, the phosphate concentration a sample can easily be determined.  

Product ID: PTK-WLR and PTK-WSR

Measure Soluble Reactive Phosphate On-Site

These kits require the use of NECi's Handheld Photometer, results are not colorimetric

Quick and Reliable Results, Easy to Use, Environmentally Benign

Use these test kit reagent refills with our handheld dual-wavelength photometer to measure phosphate levels in various types of aqueous samples, including municipal and industrial wastewater, agricultural runoff, and more.  Monitor non-point source pollution and point-source pollution on-site for accurate and reliable results in less than 30 minutes.  

If you do not yet have a handheld photometer, please view our introductory photometer package  where you can complete the bundle with a test kit pack size of your choice.  

Available in low or standard range:

• Low Range: 0.5 - 5.0 ppm Phosphate
• Standard Range: 5.0 - 20.0 Phosphate

• Obtain accurate results within 30 minutes
• Non-toxic kit contents ensure safety for users and the environment
• No laboratory experience needed
• For environmental sources of water, choose the low range test kit
• For industrial or municipal wastewater, or samples with suspected high levels of phosphate, choose the standard range test kit 
• Available in kit sizes to measure phosphate content of 5, 10, 25, or 50 samples

Product ID: PTK-SSR

Measure Bioavailable Phosphate On-Site

These kits require the use of NECi’s Handheld Photometer, results are not colorimetric

Quick and Reliable Results, Easy to Use, Environmentally Benign

This reagent refill test kit will measure 0 ppm - 20 ppm Phosphate in soil and sediment samples to be analyzed with our handheld photometer.  If you do not yet have a handheld photometer, please view our introductory photometer package where you can complete the bundle with a test kit pack size of your choice. This method is based on the Methods X article published in April 2015, Determination of phosphate in soil extracts in the field: A green chemistry enzymatic method.  This method features small sample volumes, an enzymatic reaction, and green analytical chemistry.  

Phosphate Measurement in 5 Easy Steps:

1. Collect Soil Samples, add to extraction tube and mix
2. Add drop of extraction mix to enzymatic reaction cuvette
3. Enzymes react with phosphate in sample
4. Read measurement at 360 nm using NECi's handheld photometer
5. View, track, store, and share results using any Android mobile device!

Product ID: PNP-RPK

Recombinant PNP + MESG

Optimized Balance Supply: Affinity Purified Recombinant Purine Nucleoside Phosphorylase (1.0 Unit) + MESG

NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base.  One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C.  Supplied freeze-dried with Enzyme Diluent Bulb + optimized aliquot of co-factor, MESG. 

Affinity Purified Recombinant Purine Nucleoside Phosphorylase

• CAS #: 9030-21-1
• Temperature Range in Assay: 15 – 45°C
• Physical Form:  Freeze-dried protein glass
• Expected Purity Range: 5 - 15 units/mg protein
• Recombinantly manufactured in Escherichia coli: Consistent, Reliable
• Utilizes MESG as the co-factor to accurately quantify phosphate in a sample
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives

Stability Information:

• Shelf life in freeze-dried, vacuum-sealed packets: Stable indefinitely at -20°C or lower;  Min 24 months at 4°C; Min 12 months at RT (20 – 25°C); 1 week at 30-40°C
• Shelf life in vials:  12 months + stored cool (4°C or below) and desiccated
• Shelf life in solution:  Working solution in buffer at 1 U/ml:  > 12 hours at RT and below.  Working solution containing 25% Glycerol:  minimum one year at -20°C.

Please refer to specifications for more detailed information regarding Recombinant PNP.