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This kit is supplied complete with all necessary reagents except for HPLC water. Use this kit for quantitative phosphate analysis of essentially any aqueous sample, from wastewater to soil and plant extracts. Simply extract solid samples using HPLC water before analysis. This test tube format kit is to be used with your UV capable spectrophotometer capable of reading absorbance at 360nm.
Standard Range (5 ppm - 20 ppm Phosphate) or Low Range (0.5 - 5 ppm Phosphate)
Reagents for 25 Samples or 100 Samples
When reaction is complete, the purine product yields an increase in absorbance at 360 nm in equal molar ratio to the inorganic phosphate in the reaction mixture. Using the equation of the linear regression line fitted to the set of phosphate standards, the phosphate concentration a sample can easily be determined.
Number of tests - Low Range ID - Standard Range ID
25 tests - PTK-TTLR-25 - PTK-TTSR-25
100 tests - PTK-TTLR-100 - PTK-TTSR-100
PNP Reductase Storage and Reconstitution
PNP Specification Sheet *Coming Soon*
SDS for Phosphate Test Kits
In addition to this kit, you will need a graduated cylinder, variable pipetters, a test tube vortex-type mixer, spectrophotometer capable of measuring 340 nm with UV compatible cuvettes, clean and phosphate-free 13 x 100mm test tubes, a timer, HPLC water, and ice.
NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base. One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C.
For more detailed information about this kit, please view the instruction sheet.
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Enzyme Based Analytical Chemistry
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