Product ID: PNP-1U

1.0 Unit Affinity Purified Recombinant Purine Nucleoside Phosphorylase

NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base.  One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C.  Supplied freeze-dried with Enzyme Diluent Bulb.

Affinity Purified Recombinant Purine Nucleoside Phosphorylase

• CAS #: 9030-21-1
• Temperature Range in Assay: 15 – 45°C
• Physical Form:  Freeze-dried protein glass
• Expected Purity Range: 5 - 15 units/mg protein
• Recombinantly manufactured in Escherichia coli: Consistent, Reliable
• Utilizes MESG as the co-factor to accurately quantify phosphate in a sample
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives

Stability Information:

• Shelf life in freeze-dried, vacuum-sealed packets: Stable indefinitely at -20°C or lower;  Min 24 months at 4°C; Min 12 months at RT (20 – 25°C); 1 week at 30-40°C
• Shelf life in vials:  12 months + stored cool (4°C or below) and desiccated
• Shelf life in solution:  Working solution in buffer at 1 U/ml:  > 12 hours at RT and below.  Working solution containing 25% Glycerol:  minimum one year at -20°C.

Please refer to specifications for more detailed information regarding Recombinant PNP specifications.