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Use this kit for quantification of nitrate in essentially any aqueous sample. Reactions occur on a 96-well microplate provided with the kit. This test kit is based on the enzyme Nitrate Reductase (NaR), catalyzing the reduction of nitrate to nitrite using the natural electron donor NADH. The nitrite reacts with color reagents under acidic conditions to produce a visible color. The concentration of nitrate in the original sample is determined by measuring absorbance with a microplate reader using a 540 nm filter, versus nitrate concentration in nitrate standards.
Number of tests - Low Range ID - Standard Range ID
96 tests - NTK-MPLR - NTK-MPSR
*Saltwater option is available on all product sizes and ranges.*
Standard Range Microplate Format Nitrate Test Kit Instructions
Low Range Microplate Format Nitrate Test Kit Instructions
Sample Template for Microplate Format Nitrate Test Kits
SDS for Test Tube Format and Microplate Format Nitrate Test Kits
You will need variable pipetters, a 25mL graduated cylinder, a vortex-type mixer, a microplate mixer, a microplate reader or photometer for reading absorbance at 540 nm± 20 nm, clean and nitrate-free test tubes, a timer, reagent grade water, and concentrated HCl.
Each Kit Contains:
For more detailed information about this kit, please view the instruction sheet.
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Enzyme Based Analytical Chemistry
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