Microplate Format Nitrate Test Kit: Low Range

Microplate Format Nitrate Test Kit

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Availability: In stock

$130.00

Product ID: NTK-MPLR

Measures Nitrate Within Low Range (0.05 ppm - 1.0 ppm Nitrate-N)

Analyze 96 Freshwater Samples With This Kit

Use this kit for quantification of nitrate in essentially any aqueous sample.  Reactions occur on a 96-well microplate provided with the kit.  This test kit is based on the enzyme Nitrate Reductase (NaR), catalyzing the reduction of nitrate to nitrite using the natural electron donor NADH.  The nitrite reacts with color reagents under acidic conditions to produce a visible color. The concentration of nitrate in the original sample is determined by measuring absorbance with a microplate reader using a 540 nm filter, versus nitrate concentration in nitrate standards.

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You will need variable pipetters, a 25mL graduated cylinder, a vortex-type mixer, a microplate mixer, a microplate reader or photometer for reading absorbance at 540 nm± 20 nm, clean and nitrate-free test tubes, a timer, reagent grade water, and concentrated HCl.

Each Kit Contains:

  • (1) 15mL Amber Bottle Assay Buffer (liquid)
  • (1) 15mL Amber Bottle Color Reagent No. 1 (solid)
  • (1) 15mL Amber Bottle Color Reagent No. 2 (solid)
  • (1) Tube NADH (freeze-dried)
  • (1) Tube Nitrate Reductase (freeze-dried)
  • (1) Squeeze-bulb Enzyme Diluent
  • (1) 1.5mL Tube 100 ppm Nitrate-N Nitrate Standard
  • (1) 96-well Microplate
  • (2) Pipetter Reservoir Basins
  • (6) Microcentrifuge Tubes
  • (1) 5 mL Clear Tube (for standard dilution)
  • (1) Set of Instructions

For more detailed information about this kit, please view the instruction sheet.

For technical support regarding kits, contact tech@nitrate.com

For inquiries regarding orders, or to request a custom quotation for international orders, contact sales@nitrate.com

Kits purchased to use in classroom demonstrations or projects may be eligible for discounts. Complete survey HERE.

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