Product ID: AtNaR-#U

Recombinant Plant NADH Nitrate Reductase (AtNaR)

One unit reduces 1 micromol nitrate/minute @ 37°C. 

Supplied Freeze-dried with Enzyme Diluent Bulb.

• For analysis run between 20 – 45°C
• Highly stable in working solution
• Recombinantly manufactured in Pichia pastoris: Consistent, Reliable
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity AtNaR on the market
• Utilizes NADH as the electron donor to drive nitrate reduction
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives
• Suited for 3%+ NaCl environments
• No inhibition by organic or humic acids

•    1.0 unit affinity-purified enzyme per vial, Product ID: AtNaR-1U
•    2.0 units affinity-purified enzyme per vial, Product ID: AtNaR-2U
•    3.0 units affinity-purified enzyme per vial, Product ID: AtNaR-3U
•    5.0 units affinity-purified enzyme per vial, Product ID: AtNaR-5U

Product ID: YNaR-#U

Recombinant Yeast NAD(P)H Nitrate Reductase (YNaR)

One unit reduces 1 micromol nitrate/minute @ 25°C. 

Supplied Freeze-dried with Enzyme Diluent Bulb.

• For analysis run between 2 – 30°C. 
• Highly stable in working solution
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity YNaR on the market
• Utilizes NADH as the electron donor to drive nitrate reduction
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives
• Partially inhibited by NaCl and Organic Acids and Humics, use AtNaR for these conditions

• 1.0 unit affinity-purified enzyme per vial, Product ID: YNaR-1U
• 2.0 units affinity-purified enzyme per vial, Product ID: YNaR-2U
• 3.0 units affinity-purified enzyme per vial, Product ID: YNaR-3U
• 5.0 units affinity-purified enzyme per vial, Product ID: YNaR-5U

Product ID: PNP-RPK

Recombinant PNP + MESG

Optimized Balance Supply: Affinity Purified Recombinant Purine Nucleoside Phosphorylase (1.0 Unit) + MESG

NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base.  One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C.  Supplied freeze-dried with Enzyme Diluent Bulb + optimized aliquot of co-factor, MESG. 

Affinity Purified Recombinant Purine Nucleoside Phosphorylase

• CAS #: 9030-21-1
• Temperature Range in Assay: 15 – 45°C
• Physical Form:  Freeze-dried protein glass
• Expected Purity Range: 5 - 15 units/mg protein
• Recombinantly manufactured in Escherichia coli: Consistent, Reliable
• Utilizes MESG as the co-factor to accurately quantify phosphate in a sample
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives

Stability Information:

• Shelf life in freeze-dried, vacuum-sealed packets: Stable indefinitely at -20°C or lower;  Min 24 months at 4°C; Min 12 months at RT (20 – 25°C); 1 week at 30-40°C
• Shelf life in vials:  12 months + stored cool (4°C or below) and desiccated
• Shelf life in solution:  Working solution in buffer at 1 U/ml:  > 12 hours at RT and below.  Working solution containing 25% Glycerol:  minimum one year at -20°C.

Please refer to specifications for more detailed information regarding Recombinant PNP.

Product ID: PNP-#U

Variable Units Affinity Purified Recombinant Purine Nucleoside Phosphorylase

NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base.  One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C. Supplied freeze-dried with Enzyme Diluent Bulb.

Affinity Purified Recombinant Purine Nucleoside Phosphorylase

• CAS #: 9030-21-1
• Temperature Range in Assay: 15 – 45°C
• Physical Form:  Freeze-dried protein glass
• Expected Purity Range: 5 - 15 units/mg protein
• Recombinantly manufactured in Escherichia coli: Consistent, Reliable
• Utilizes MESG as the co-factor to accurately quantify phosphate in a sample
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives

Stability Information:

• Shelf life in freeze-dried, vacuum-sealed packets: Stable indefinitely at -20°C or lower;  Min 24 months at 4°C; Min 12 months at RT (20 – 25°C); 1 week at 30-40°C
• Shelf life in vials:  12 months + stored cool (4°C or below) and desiccated
• Shelf life in solution:  Working solution in buffer at 1 U/ml:  > 12 hours at RT and below.  Working solution containing 25% Glycerol:  minimum one year at -20°C.

Please refer to specifications for more detailed information regarding Recombinant PNP specifications.

•    1.0 unit affinity-purified enzyme per vial, Product ID: PNP-1U
•    2.0 units affinity-purified enzyme per vial, Product ID: PNP-2U
•    3.0 units affinity-purified enzyme per vial, Product ID: PNP-3U
•    5.0 units affinity-purified enzyme per vial, Product ID: PNP-5U

Product ID: AtNaR-RPk

Previously sold as:  NaR-PkAt

Superior Stock AtNaR + NADH Packs For Your Laboratory

Packs include 1.0 unit of AtNaR plus calibrated quantities of reaction cofactors (freeze-dried form)

Recombinant Plant NADH Nitrate Reductase (AtNaR)

• For analysis run between 20 – 45°C. 
• Highly stable in working solution
• Each pack sufficient for 20-50 assays, depending on your assay design
• Manufactured in Pichia pastoris 
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity AtNaR on the market
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives
• Suited for 3%+ NaCl environments

Please refer to specifications for more detailed information regarding AtNaR

Product ID: YNaR-RPk

Previously sold as:  NaR-PkY

1.0 Unit YNaR + NADH Aliquot Packs for your laboratory

Packs include 1.0 unit of YNaR plus calibrated quantities of reaction cofactors (freeze-dried form)

Recombinant Yeast NAD(P)H Nitrate Reductase (YNaR)

• For analysis run between 2 – 30°C. 
• Highly stable in working solution
• Each pack sufficient for 20-50 assays, depending on your assay design
• Manufactured in Pichia pastoris 
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity YNaR on the market
• Utilizes NADH as the electron donor to drive nitrate reduction
• Small sample size in relation to total assay volume
• Little sample preparation required
• High specificity reduces false positives
• Partially inhibited by NaCl and Organic Acids and Humics, use AtNaR for these conditions

Please refer to specifications for more detailed information regarding YNaR

Product ID: NRPk-AND-1At

Previously sold as: DA-NPk-901

Compatible with Tabletop Discrete Analyzers measuring Nitrate by NADH disappearance such as Hitachi 717

AtNaR & NADH Reagents for 75 Samples

Packs include 1.0 unit of AtNaR (freeze-dried form), 2.4mg NADH (solid form), and 1 vial of enzyme diluent

Contact us to design a kit tailored to your machine (if not listed) for no extra charge.

Recombinant Plant NADH Nitrate Reductase (AtNaR)

• For analysis run between 20 – 45°C. 
• Highly stable in working solution
• Manufactured in Pichia pastoris 
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity AtNaR on the market
• High specificity reduces false positives
• Suited for 3%+ NaCl environments

For more detailed information about this kit, please refer to the instruction sheet.

Product ID: NRPk-DA-1At

Previously sold as: DA-ARK-1 OIP

Compatible with Tabletop Discrete Analyzers such as Unity (Westco) Smartchem, OI Analytical Discrete Analyzers, Thermo Scientific™ Gallery™, and other machines with a similar format.

AtNaR & NADH Reagents for 50-200 Samples

Packs include 1.0 unit of AtNaR (freeze-dried form), 0.7 mg NADH (solid form), and 1 vial of enzyme diluent

Contact us to design a kit tailored to your machine (if not listed) for no extra charge.

Recombinant Plant NADH Nitrate Reductase (AtNaR)

• For analysis run between 20 – 45°C. 
• Highly stable in working solution
• Manufactured in Pichia pastoris 
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity AtNaR on the market
• High specificity reduces false positives
• Suited for 3%+ NaCl environments

For more detailed information about this kit, please refer to the instruction sheet.

Product ID: NRPk-HDA-3At

Previously sold as:  DA-ARK-1

Compatible with High Capacity Stand Alone Discrete Analyzers (Thermo Scientific™  Aquakem™)

AtNaR & NADH Reagents for minimum 300 assays

Packs include 3.0 units of AtNaR (freeze-dried form), 2mg NADH (solid form), and 1 vial of enzyme diluent

Contact us to design a kit tailored to your machine (if not listed) for no extra charge.

Recombinant Plant NADH Nitrate Reductase (AtNaR)

• For analysis run between 20 – 45°C
• Highly stable in working solution
• Manufactured in Pichia pastoris 
• Shelf-life of up to 6 months at room temperature
• Highest Specific Activity AtNaR on the market
• High specificity reduces false positives
• Suited for 3%+ NaCl environments

For more detailed information about this kit, please refer to the instruction sheet.

More information about Thermo Scientific Discrete Analysis Systems can be found at www.thermofisher.com/discreteanalysis