Using Enzymes for Regulatory Compliance Reporting


For When Accurate Data Matters Most

Nitrate is a “primary contaminant” under the EPA’s Safe Drinking Water and Clean Water Acts. Phosphate measurement is required by many NPDES permits. Both nitrate and phosphate are important plant nutrients, but cause serious problems when too much ends up in our water. The US EPA has set the maximum allowable level for nitrate at 10.0ppm Nitrate-N.

Various multi-laboratory validation studies have been conducted comparing the performance of the nitrate reductase method for nitrate-n analysis. These rigorous validation studies have resulted in approval of the enzymatic method for regulatory compliance reporting for laboratories using methods dictated by agencies such as the U.S. EPA, ASTM International, U.S. Geological Survey, and Standard Methods.

The enzymatic nitrate-n analysis method using nitrate reductase has been verified for accuracy, precision, and sensitivity to provide a robust and trusted method for regulated commercial laboratories worldwide. The nitrate reductase method is designed in multiple formats for a wide range of applications - manual test tube, microplate, automated instruments, simplified on-site, and more.


REGULATORY AGENCY METHOD # REVISION DATE METHOD TITLE TNI CODE
U.S. Geological Survey USGS I-2547-11 2011 Standard Level Colorimetric Determination of Nitrate plus Nitrite by Enzymatic Reduction, Automated Discrete Analyzer Method 40005285
U.S. Geological Survey USGS I-2548-11 2011 Low Level Colorimetric Determination of Nitrate plus Nitrite by Enzymatic Reduction, Automated Discrete Analyzer Method 40005296
ASTM International  ASTM D7781-14  2014 Nitrate-Nitrite in Water by Nitrate Reductase 30034107
U.S. EPA CWA* EPA ATP Case No. N07-0003 *Anticipated 2017 Nitrate Reductase Nitrate-Nitrogen Analysis N/A
U.S EPA SDWA EPA NECi 2016a 2016 Method for Nitrate Reductase Nitrate-Nitrogen Analysis of Drinking Water  N/A

IMPORTANT LINKS & DOCUMENTS

U.S. EPA
ASTM International
U.S. Geological Survey

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ON-SITE TEST KITS

Complete with everything needed for quick and easy analysis anywhere. These user friendly on-the-go kits are pre-measured and pre-calculated for results, no laboratory or experience needed.

LABORATORY TEST KITS

Complete with most necessary reagents, including buffers, standards, color reagents, co-factors. Requires a basic laboratory equipped with ice, labware, pipettes, and photometer or microplate reader.

ENZYME REAGENT PACKS

Freeze-dried enzyme + reconstitution diluent included, available with or without optimized co-factor. Also available formatted for discrete analyzers, flow injection analyzers, and other automated instruments.