{"product_id":"atnar-nitrate-reductase-reagents-kit-for-flow-injection-continuous-flow-and-segmented-flow-analyzers","title":"AtNaR Nitrate Reductase Reagents Kit for Flow Injection, Continuous Flow, and Segmented Flow Analyzers","description":"\u003ch2\u003eEPA-Compliant, Cadmium-Free Nitrate Reagent Kit for Flow Injection, Continuous Flow, and Segmented Flow Analyzers\u003c\/h2\u003e\n\u003cp\u003eAtNaR Nitrate Reductase Reagent Packs were developed by NECi in conjunction with the \u003cstrong\u003eUnited States Geological Survey (USGS)\u003c\/strong\u003e to replace toxic cadmium used in automated colorimetric nitrate determination methods. The enzymatic nitrate reduction approach aligns with procedures referenced by the \u003cstrong\u003eU.S. Environmental Protection Agency (EPA)\u003c\/strong\u003e under \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/MUR_2017-PUBLISHED_FED_REGISTER.pdf\" title=\"EPA 40 CFR Part 136\"\u003e\u003cstrong\u003e40 CFR Part 136\u003c\/strong\u003e\u003c\/a\u003e for nitrate determination in wastewater and environmental water samples. This method is also included in the California \u003cstrong\u003eEnvironmental Laboratory Accreditation Program (ELAP)\u003c\/strong\u003e Field of Accreditation (FOA) tables, enabling laboratories to pursue accreditation using this cadmium-free method.\u003c\/p\u003e\n\u003cp\u003eThis non-toxic, environmentally benign alternative provides reliable nitrate reduction using water-soluble reagents optimized for flow-based analyzers.\u003c\/p\u003e\n\u003ch2\u003eProduct Format\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAtNaR Nitrate Reductase Reagent Pack for FIA\/CFA\/SFA Analyzers\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eAnimal origin free\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eDesigned for \u003cstrong\u003eLACHAT QuikChem® flow injection and continuous flow analyzers\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003eMay be compatible with other flow injection, continuous flow, and segmented flow analyzers with similar specifications\u003c\/li\u003e\n\u003cli\u003eContact us to design a kit tailored to your specific instrument (if not listed) at no extra charge\u003c\/li\u003e\n\u003cli\u003eThis kit contains only enzyme and co-factor. You will also need \u003ca title=\"Assay Buffer\" href=\"\/products\/assay-buffer\"\u003eassay buffer\u003c\/a\u003e and Griess reagents to run the assay. \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReaction Principle\u003c\/h2\u003e\n\u003cp\u003eAtNaR catalyzes the reduction of \u003cstrong\u003enitrate (NO₃⁻) to nitrite (NO₂⁻)\u003c\/strong\u003e using \u003cstrong\u003eNADH as the electron donor\u003c\/strong\u003e.\u003cbr\u003eThe generated nitrite can then be reacted with Griess reagents and quantified using standard colorimetric detection methods.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe \u0026amp; Non-Toxic:\u003c\/strong\u003e Eliminates hazardous cadmium from your laboratory workflow\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnvironmentally Friendly:\u003c\/strong\u003e All reagents are water-soluble and environmentally benign\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExceptional Stability:\u003c\/strong\u003e Up to 6 months shelf life at room temperature, highly stable in working solution\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuperior Performance:\u003c\/strong\u003e Highest specific activity AtNaR on the market with high specificity to reduce false positives\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWide Operating Range:\u003c\/strong\u003e Effective for analysis run between 20 – 45°C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSalt Tolerant:\u003c\/strong\u003e Suited for 3%+ NaCl environments\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCost-Effective:\u003c\/strong\u003e Stable working solutions minimize waste and reagent consumption\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuality Assured:\u003c\/strong\u003e Recombinant enzyme manufactured in \u003cem\u003ePichia pastoris\u003c\/em\u003e under strict QA\/QC procedures for lot-to-lot consistency and reliability\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS Number:\u003c\/strong\u003e 9013-03-0\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePhysical Form:\u003c\/strong\u003e Freeze-dried protein glass\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecific Activity:\u003c\/strong\u003e 5-15 units\/mg enzyme protein\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected Purity Range:\u003c\/strong\u003e Greater than 95%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTemperature Range in Assay:\u003c\/strong\u003e 15-45°C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eManufacturing:\u003c\/strong\u003e Recombinantly manufactured in \u003cem\u003ePichia pastoris\u003c\/em\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eIdeal for laboratories performing high-throughput nitrate analysis in:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDrinking water quality testing and regulatory compliance\u003c\/li\u003e\n\u003cli\u003eEnvironmental monitoring and surface water analysis\u003c\/li\u003e\n\u003cli\u003eWastewater treatment facility testing\u003c\/li\u003e\n\u003cli\u003eGroundwater contamination assessment\u003c\/li\u003e\n\u003cli\u003eAgricultural runoff and soil nitrate monitoring\u003c\/li\u003e\n\u003cli\u003eResearch and development laboratories\u003c\/li\u003e\n\u003cli\u003eGovernment and regulatory testing facilities\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eAnalyzer Compatibility\u003c\/h2\u003e\n\u003cp\u003eThese kits are designed for \u003cstrong\u003eLACHAT QuikChem® flow injection and continuous flow analyzers\u003c\/strong\u003e and may be compatible with other flow injection, continuous flow, and segmented flow analyzers with similar specifications. Please inquire about reagent packs with detailed instructions for other flow-based instruments, discrete analyzers, and other automated laboratory instruments.\u003c\/p\u003e\n\u003ch2\u003eKit Contents\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFIA\/CFA\/SFA Kit:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e3.0 units AtNaR (freeze-dried)\u003c\/li\u003e\n\u003cli\u003e8.0 mg NADH (solid form)\u003c\/li\u003e\n\u003cli\u003e1 vial enzyme diluent\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage Conditions \u0026amp; Shelf Life Information*\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e*Shelf life guaranteed if stored as directed below\u003c\/em\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #000; padding: 8px;\"\u003e\u003cbr\u003e\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #000; padding: 8px;\"\u003e-80°C\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #000; padding: 8px;\"\u003e-20°C\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #000; padding: 8px;\"\u003e4°C\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #000; padding: 8px;\"\u003eRT: 20°C – 25°C\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #000; padding: 8px;\"\u003eAbove 40°C\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e\u003cstrong\u003eFreeze-Dried\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e3+ Years\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e2+ Years\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e1+ Year\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e6 months\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003eUp to 1 week\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e\u003cstrong\u003eIn Solution*\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e1+ Year\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003e6+ months\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003eUp to 1 week\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003eNot recommended\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #000; padding: 8px;\"\u003eNot recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Enzyme provided with enzyme diluent working solution, contains 25% glycerol\u003c\/p\u003e\n\u003ch2\u003eCompatible Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"products\/assay-buffer-100-ml\" title=\"Assay Buffer (100 mL)\" rel=\"noopener\" target=\"_blank\"\u003eAssay Buffer (100 mL)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"products\/nadh-for-nitrate-reductase-assays\" title=\"NADH for Nitrate Reductase Assays\" rel=\"noopener\" target=\"_blank\"\u003eNADH for Nitrate Reductase Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Contact Us\" href=\"\/pages\/contact\" target=\"_blank\"\u003eContact us\u003c\/a\u003e to inquire about bulk quantity discounts or wholesale orders, or to request a quote for international orders. Custom unit sizes available by request.\u003c\/p\u003e\n\u003ch2\u003eResources \u0026amp; Downloads\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/LaChat_QuikChem_FIA_Brackish_Water_Nitrate_Method.pdf\" title=\"LaChat QuickChem Flow Injection Analysis Brackish Water Nitrate Method\" rel=\"noopener\" target=\"_blank\"\u003eLaChat QuickChem Flow Injection Analysis Brackish Water Nitrate Method\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/LaChat_QuikChem_FIA_Soil_Nitrate_Method.pdf\" title=\"LaChat QuickChem Flow Injection Analysis Soil Nitrate Method\" rel=\"noopener\" target=\"_blank\"\u003eLaChat QuickChem Flow Injection Analysis Soil Nitrate Method\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/LaChat_QuikChem_FIA_Water_Nitrate_Method.pdf\" title=\"LaChat QuickChem Flow Injection Analysis Water Nitrate Method\" rel=\"noopener\" target=\"_blank\"\u003eLaChat QuickChem Flow Injection Analysis Water Nitrate Method\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/SDS_Reagent_Packs.pdf\" title=\"SDS Nitrate Reductase Reagent Packs\" rel=\"noopener\" target=\"_blank\"\u003eSDS for Reagent Packs\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/NaR_Storage_and_Reconstitution.pdf\" title=\"Nitrate Reductase Enzyme Storage and Reconstitution\" rel=\"noopener\" target=\"_blank\"\u003eNitrate Reductase Storage and Reconstitution\u003c\/a\u003e\u003c\/p\u003e","brand":"NECi","offers":[{"title":"Default Title","offer_id":54055487177068,"sku":"NRPk-FIA-3At","price":90.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0972\/5830\/7948\/files\/NRPk-HDA-3At.jpg?v=1773174585","url":"https:\/\/nitrate.com\/products\/atnar-nitrate-reductase-reagents-kit-for-flow-injection-continuous-flow-and-segmented-flow-analyzers","provider":"NECi","version":"1.0","type":"link"}