Products for Phosphate Analysis

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  • Photometer Pack Contents

    Handheld Photometer Introductory Package

    From: $270.00

    To: $836.00

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    torchlake photometer          

    Product ID: PHOT-PACK

    For Easy, On-Site Analysis of Nitrate and Phosphate in a Variety of Sample Types

    NECi's handheld photometer coupled with enzyme-based on-site test kits offers accurate and reliable quality data anywhere.  The photometer interfaces with Android mobile devices via Bluetooth technology to provide digital results which are stored and easily exported for further analysis & collaboration.

    The computational software is included in the free Android mobile application, with options to track sample collection date, time, and location.  The device is enclosed in a durable 3-D printed case made of PLA, a biodegradable thermoplastic from renewable resources.

    • Portable, Rechargeable, Durable
    • User-friendly Mobile App Interface
    • Data Tracking & Storage on Android Devices
    • Wireless Interface to Mobile Devices
    • GPS + Sample Collection Time Features
    • Easy Export via CSV for Further Analysis
    • Enzymatic Test Kits for Quality Data & Non-Hazardous Analysis
    • Small Reaction Volumes Minimize Waste
    • True Optical Bench + Cuvettes for Reliable Results
    • Quick, Economical Analysis for Many Applications

    Choose Your Test Kit Pack for 10, 25, or 50 Samples*

    Parameter

    Range

    Method

    Nitrate-N, Standard Range Water 0.5 - 10 ppm Enzymatic (nitrate reductase)
    Nitrate-N, Low Range Water 0.01 - 2.0 ppm Enzymatic (nitrate reductase)
    Nitrate-N, Soil 0 - 50 ppm Enzymatic (nitrate reductase)
    Nitrate-N, Plant Petioles 0 - 1000 ppm Enzymatic (nitrate reductase)
    Nitrate-N, Green & Dry Forage 0 - 2000 ppm Enzymatic (nitrate reductase)
    Phosphate (SRP), Standard Range, Water or Soil 5 - 20 ppm Enzymatic (PNP)
    Phosphate (SRP), Low Range 0.5 - 5 ppm Enzymatic (PNP)

    *Larger pack sizes available upon request

  • Test Tube Format Phosphate Test Kits

    Test Tube Format Phosphate Test Kits

    $45.00
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    Product ID: PTK-TTLR or PTK-TTSR

    This kit is supplied complete with all necessary reagents except for HPLC water.  Use this kit for quantitative phosphate analysis of essentially any aqueous sample, from wastewater to soil and plant extracts.  Simply extract solid samples using HPLC water before analysis.  This test tube format kit is to be used with your UV capable spectrophotometer capable of reading absorbance at 360nm.    

    Quantitates Phosphate in Complex Mixtures

    Choose kit type using choices below, or contact us for a custom kit fit for your application:

    • Standard Range (5 ppm - 20 ppm Phosphate) or Low Range (0.5 - 5 ppm Phosphate)

    • Reagents for 25 Samples or 100 Samples


    PNP Assay

    When reaction is complete, the purine product yields an increase in absorbance at 360 nm in equal molar ratio to the inorganic phosphate in the reaction mixture.  Using the equation of the linear regression line fitted to the set of phosphate standards, the phosphate concentration a sample can easily be determined.  

  • Water Phosphate Test Kits

    Water Phosphate Test Kits

    $40.00
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    Product ID: PTK-WLR and PTK-WSR

    Measure Soluble Reactive Phosphate On-Site

    These kits require the use of NECi's Handheld Photometer, results are not colorimetric

    Quick and Reliable Results, Easy to Use, Environmentally Benign

    Use these test kit reagent refills with our handheld dual-wavelength photometer to measure phosphate levels in various types of aqueous samples, including municipal and industrial wastewater, agricultural runoff, and more.  Monitor non-point source pollution and point-source pollution on-site for accurate and reliable results in less than 30 minutes.  

    If you do not yet have a handheld photometer, please view our introductory photometer package complete with a test kit pack size of your choice.  

    Available in low or standard range:

    • Low Range: 0.5 - 5.0 ppm Phosphate
    • Standard Range: 5.0 - 20.0 Phosphate

     

    • Obtain accurate results within 30 minutes
    • Non-toxic kit contents ensure safety for users and the environment
    • No laboratory experience needed
    • For environmental sources of water, choose the low range test kit
    • For industrial or municipal wastewater, or samples with suspected high levels of phosphate, choose the standard range test kit 
    • Available in kit sizes to measure phosphate content of 5, 10, 25, or 50 samples
  • Soil Phosphate Test Kit

    Soil Phosphate Test Kit

    $40.00
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    Product ID: PTK-SSR

    Measure Bioavailable Phosphate On-Site

    These kits require the use of NECi’s Handheld Photometer, results are not colorimetric

    Quick and Reliable Results, Easy to Use, Environmentally Benign

    This reagent refill test kit will measure 0 ppm - 20 ppm Phosphate in soil and sediment samples to be analyzed with our handheld photometer.  If you do not yet have a handheld photometer, please view our introductory photometer package complete with a test kit for 10 samples. This method is based on the Methods X article published in April 2015, Determination of phosphate in soil extracts in the field: A green chemistry enzymatic method.  This method features small sample volumes, an enzymatic reaction, and green analytical chemistry.  


     

    Phosphate Measurement in 5 Easy Steps:

    1. Collect Soil Samples, add to extraction tube and mix
    2. Add drop of extraction mix to enzymatic reaction cuvette
    3. Enzymes react with phosphate in sample
    4. Read measurement at 360 nm using NECi's handheld photometer
    5. View, track, store, and share results using any Android mobile device!
  • PNP +  MESG Reagent Pack

    PNP + MESG Reagent Pack

    $25.00
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    Product ID: PNP-RPK

    Recombinant PNP + MESG

    Optimized Balance Supply: Affinity Purified Recombinant Purine Nucleoside Phosphorylase (1.0 Unit) + MESG

    NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base.  One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C.  Supplied freeze-dried with Enzyme Diluent Bulb + optimized aliquot of co-factor, MESG. 

    Affinity Purified Recombinant Purine Nucleoside Phosphorylase

    • CAS #: 9030-21-1
    • Temperature Range in Assay: 15 – 45°C
    • Physical Form:  Freeze-dried protein glass
    • Expected Purity Range: 5 - 15 units/mg protein
    • Recombinantly manufactured in Escherichia coli: Consistent, Reliable
    • Utilizes MESG as the co-factor to accurately quantify phosphate in a sample
    • Small sample size in relation to total assay volume
    • Little sample preparation required
    • High specificity reduces false positives

    Stability Information:

    • Shelf life in freeze-dried, vacuum-sealed packets: Stable indefinitely at -20°C or lower;  Min 24 months at 4°C; Min 12 months at RT (20 – 25°C); 1 week at 30-40°C
    • Shelf life in vials:  12 months + stored cool (4°C or below) and desiccated
    • Shelf life in solution:  Working solution in buffer at 1 U/ml:  > 12 hours at RT and below.  Working solution containing 25% Glycerol:  minimum one year at -20°C.

    Please refer to specifications for more detailed information regarding Recombinant PNP.

  • Purine Nucleoside Phosphorylase: PNP

    Purine Nucleoside Phosphorylase: PNP

    $20.00
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    Product ID: PNP-#U

    Variable Units Affinity Purified Recombinant Purine Nucleoside Phosphorylase

    NECi Recombinant PNP catalyzes Ortho-Phosphate dependent transfer to ribose with release of free purine base.  One unit produces 1 µmole of hypoxanthine from inoside per minute, at pH 8.0 and 22°C. Supplied freeze-dried with Enzyme Diluent Bulb.

    Affinity Purified Recombinant Purine Nucleoside Phosphorylase

    • CAS #: 9030-21-1
    • Temperature Range in Assay: 15 – 45°C
    • Physical Form:  Freeze-dried protein glass
    • Expected Purity Range: 5 - 15 units/mg protein
    • Recombinantly manufactured in Escherichia coli: Consistent, Reliable
    • Utilizes MESG as the co-factor to accurately quantify phosphate in a sample
    • Small sample size in relation to total assay volume
    • Little sample preparation required
    • High specificity reduces false positives

    Stability Information:

    • Shelf life in freeze-dried, vacuum-sealed packets: Stable indefinitely at -20°C or lower;  Min 24 months at 4°C; Min 12 months at RT (20 – 25°C); 1 week at 30-40°C
    • Shelf life in vials:  12 months + stored cool (4°C or below) and desiccated
    • Shelf life in solution:  Working solution in buffer at 1 U/ml:  > 12 hours at RT and below.  Working solution containing 25% Glycerol:  minimum one year at -20°C.

    Please refer to specifications for more detailed information regarding Recombinant PNP specifications.

    Available in the following Unit sizes:
    •    1.0 unit affinity-purified enzyme per vial, Product ID: PNP-1U
    •    2.0 units affinity-purified enzyme per vial, Product ID: PNP-2U
    •    3.0 units affinity-purified enzyme per vial, Product ID: PNP-3U
    •    5.0 units affinity-purified enzyme per vial, Product ID: PNP-5U

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