Laboratory Test Kits

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  • Test Tube Format Nitrate Test Kits

    Test Tube Format Nitrate Test Kits

    $45.00
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    Product ID: NTK-TTLR or NTK-TTSR

    Use this kit for quantitative nitrate analysis of essentially any aqueous sample, from wastewater to soil and plant extracts.  This test tube format kit is to be used with your colorimeter or spectrophotometer capable of reading at 540nm± 20nm.  NECi's recombinant Nitrate Reductase drives all Nitrate to Nitrite using NADH, which reacts with color reagents under acidic conditions to produce a visible color.  

    Nitrate Reductase Based Test Tube Format Kits Available in:

    • 25 Sample or 100 Sample Analysis 

    • Low Range (0.05 ppm-1.0 ppm Nitrate-N) or Standard Range (1.0 ppm-10.0 ppm Nitrate-N)

    • Freshwater or Saltwater Sample Analysis

    NECi Nitrate Reductase (AtNaR):

    • Recombinant AtNaR fermented according to strict QA/QC procedures with reagent grade water and analytical grade sugars and salts, ensuring lot-to-lot consistency, reliablility, and stability 

    • Purified to Homegeneity by Immobilized Metal Affinity Chromatography Guarantees Greater than 95% Purity

    • Freeze Dried Stable Indefinitely at -20°C, 2 Years at 4°C, 1 Year at 25°C (RT)

    • Stable in Working Solution for 1 Year at -20°C, 2 Weeks at 4°C, 8 Hours at 25°C (RT)

    • Small Sample Volume and Enzyme Reagents Offer Sustainability and No Hassle Shipping, Storage and Disposal for Your Laboratory 

  • Microplate Format Nitrate Test Kits

    Microplate Format Nitrate Test Kits

    $125.00
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    Product ID: NTK-MPLR or NTK-MPSR

    Use this kit for quantification of nitrate in essentially any aqueous sample.  Reactions occur on a 96-well microplate provided with the kit.  This test kit is based on the enzyme Nitrate Reductase (NaR), catalyzing the reduction of nitrate to nitrite using the natural electron donor NADH.  The nitrite reacts with color reagents under acidic conditions to produce a visible color. The concentration of nitrate in the original sample is determined by measuring absorbance with a microplate reader using a 540 nm filter, versus nitrate concentration in nitrate standards.

    Nitrate Reductase Based Microplate Test Kit Available in:

    • Standard Range (1.0 ppm - 10.0 ppm Nitrate-N) or Low Range (0.5 ppm - 1.0 ppm Nitrate-N)
    • Freshwater or Saltwater Analysis
  • Test Tube Format Phosphate Test Kits

    Test Tube Format Phosphate Test Kits

    $45.00
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    Product ID: PTK-TTLR or PTK-TTSR

    This kit is supplied complete with all necessary reagents except for HPLC water.  Use this kit for quantitative phosphate analysis of essentially any aqueous sample, from wastewater to soil and plant extracts.  Simply extract solid samples using HPLC water before analysis.  This test tube format kit is to be used with your UV capable spectrophotometer capable of reading absorbance at 360nm.    

    Quantitates Phosphate in Complex Mixtures

    Choose kit type using choices below, or contact us for a custom kit fit for your application:

    • Standard Range (5 ppm - 20 ppm Phosphate) or Low Range (0.5 - 5 ppm Phosphate)

    • Reagents for 25 Samples or 100 Samples


    PNP Assay

    When reaction is complete, the purine product yields an increase in absorbance at 360 nm in equal molar ratio to the inorganic phosphate in the reaction mixture.  Using the equation of the linear regression line fitted to the set of phosphate standards, the phosphate concentration a sample can easily be determined.  

  • Nitrate Test Kit by NADH Disappearance: 100 Sample Analysis

    Nitrate Test Kits by NADH Disappearance

    $50.00
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    Product ID: NTK-ND-25 and NTK-ND-100

    Use this kit to obtain quantitative nitrate data within standard range by measuring NADH Disappearance.  Measures nitrate content in food extracts and other aqueous extracts or samples.  Confirmed by NECi to provide accurate results when used in conjunction with Carrez's Reagent in meat, milk, and whey.  Use your standard food sample preparation method or see product documents for more information.

    NADH Disappearance Nitrate Reductase Based Test Kit Available In:

    • Standard Range (0.5 ppm - 10.0 ppm Nitrate-N)
    • Choose from Analysis of 25 or 100 Samples

    NECi Nitrate Reductase (AtNaR):

    • Recombinant AtNaR fermented according to strict QA/QC procedures with reagent grade water and analytical grade sugars and salts, ensuring lot-to-lot consistency, reliablility, and stability 

    • Purified to Homegeneity by Immobilized Metal Affinity Chromatography Guarantees Greater than 95% Purity

    • Freeze Dried Stable Indefinitely at -20°C, 2 Years at 4°C, 1 Year at 25°C (RT)

    • Stable in Working Solution for 1 Year at -20°C, 2 Weeks at 4°C, 8 Hours at 25°C (RT)

    • Small Sample Volume and Enzyme Reagents Offer Sustainability and No Hassle Shipping, Storage and Disposal for Your Laboratory 

  • Nitrate Reductase Activity Assay Kit

    Nitrate Reductase Activity Assay Kit

    $150.00
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    Product ID: NaRA-10

    This kit enables you to prepare extracts of 10 samples and to assay these extracts for NADH: Nitrate Reductase (NaR) activity in roots, stems, leaves, whole plants, fungal or microbial extracts.  The enzyme Nitrate Reductase (NaR) catalyzes the reduction of Nitrate to Nitrite using the natural electron donor NADH.  The nitrite reacts with color reagents (dyes) under acidic conditions to produce a visible magenta color.  The color produced is directly proportional to the nitrate concentration.  The quantity of nitrate reduced in the reaction time tells you how much active nitrate reductase is present in your sample.

    The Enzyme Commission definition of NaR activity is expressed as micromoles of nitrite produced per minute per ml of enzyme (µmol nitrite formed/ min/ ml of extract), in a buffer at pH 7.5 at 30°C.  We provide a Nitrate Reductase Activity Standard (YNaR) at 1.0 Unit per milliliter (1.0 U reduces one µmol nitrate per minute).   You will use this YNaR standard to determine the NaR activity in your samples.

    Use These Assay Kits in:

    •     Plant Physiology and Ecology Research Labs
    •     Biochemistry Research Labs
    •     Environmental Research on Atmospheric Deposit
    •     Agronomy and Nutrient Status Studies
    •     Teaching Courses

    Note: The assay will not detect NADPH: or methyl viologen: NaR activities

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