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This kit enables you to prepare extracts of 10 samples and to assay these extracts for NADH: Nitrate Reductase (NaR) activity in roots, stems, leaves, whole plants, fungal or microbial extracts. The enzyme Nitrate Reductase (NaR) catalyzes the reduction of Nitrate to Nitrite using the natural electron donor NADH. The nitrite reacts with color reagents (dyes) under acidic conditions to produce a visible magenta color. The color produced is directly proportional to the nitrate concentration. The quantity of nitrate reduced in the reaction time tells you how much active nitrate reductase is present in your sample.
The Enzyme Commission definition of NaR activity is expressed as micromoles of nitrite produced per minute per ml of enzyme (µmol nitrite formed/ min/ ml of extract), in a buffer at pH 7.5 at 30°C. We provide a Nitrate Reductase Activity Standard (YNaR) at 1.0 Unit per milliliter (1.0 U reduces one µmol nitrate per minute). You will use this YNaR standard to determine the NaR activity in your samples.
Note: The assay will not detect NADPH: or methyl viologen: NaR activities
Nitrate Reductase Activity Test Kit Instructions
SDS for Nitrate Reductase Activity Kits
Each Kit Contains:
For more detailed information about this kit, please view the instruction sheet.
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Enzyme Based Analytical Chemistry
Test kits for laboratories, agriculture, homeowners, and more
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