NECi Custom Protein/Enzyme Expression Service
 

      The Nitrate Elimination Co., Inc.
   Green Chemistry for Nitrate Detection
                              Biotechnology People Can Use

Revised and Updated: 12/18/2008                                                             1.888.NITRATE  www.nitrate.com

NECi Fermenter growing Pichia pastoris expressing Superior Stock NaR

 

NECi produces NaR and other enzymes for commercial sale at its lab in Lake Linden, Michigan

NECi will produce your protein of interest using standard protocols developed in-house with complete confidentiality.

Contact Bill with your questions concerning protein expression:
bill@nitrate.com

NECi produces proteins in Pichia pastoris.

Publications and Patent Applications of Wilbur H. (Bill) Campbell and colleagues where Pichia Expression was used to produce Superior Stock NaR and its catalytically active fragments including biochemical studies of various enzyme forms produced in Pichia.

Some of these pubs are available on the web as shown by links.

Campbell, WH, T Kinnunen-Skidmore, MJ Brodeur-Campbell & ER Campbell (2004) New and improved nitrate reductase for enzymatic nitrate analysis.  American Laboratory 22(10): 12. read it

Barbier, GG, JC Joshi, ER Campbell & WH Campbell (2004) Purification and biochemical characterization of simplified eukaryotic nitrate reductase expressed in Pichia pastoris.  Protein Expression and Purification. 37: 61-71. Read this paper

Skipper L, Campbell WH, Mertens JA, Lowe DJ. (2001) Pre-steady-state kinetic analysis of recombinant Arabidopsis NADH:nitrate reductase: rate-limiting processes in catalysis. Journal of Biological Chemistry, 2001 Jul 20;276(29):26995-7002.

Mertens JA, Shiraishi N, Campbell WH. (2000) Recombinant expression of molybdenum reductase fragments of plant nitrate reductase at high levels in Pichia pastoris. Plant Physiology 2000 Jun;123(2):743-56.

Su W, Mertens JA, Kanamaru K, Campbell WH, Crawford NM. (1997) Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris. Plant Physiology. 1997 Nov;115(3):1135-43.

Barbier, GG, Campbell, WH (2000) Expression of Pichia angusta nitrate reductase (YNR1) in Pichia pastoris.  Current Topics in Gene Expression Systems: Program and Abstracts, Abstract PY 4, p. 64.

Ratnam, K., N. Shiraishi, Wilbur H. Campbell, and R. Hille (1997) Spectroscopic and kinetic characterization of the recombinant cytochrome c reductase fragment of nitrate reductase: identification of the rate limiting catalytic step. Journal of Biological Chemistry, 272: 2122-2128.

Simplified eukaryotic nitrate reductase. Provisional patent application filed by The Nitrate Elimination Co.,  Inc., on behalf of Wilbur H. Campbell, Guillaume G. Barbier, and Ellen  R. Campbell. December 14, 2001, Serial Number – 60/340,237.

Simplified eukaryotic nitrate reductase. International patent application filed by The Nitrate Elimination Co.,  Inc., on behalf of Wilbur H. Campbell, Guillaume G. Barbier, and Ellen  R. Campbell, serial number PCT-US02-39341 (international patent publication WO 03/052063, June 26, 2003).